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KMID : 0378119840110010135
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Chungnam Medical Journal
1984 Volume.11 No. 1 p.135 ~ p.142
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Studies on The Improved Detection of Acid-Fast Bacilli from Sputum Sediments by Filter Smear Method
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Abstract
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Sputum specimens from the patients with active cases of tuberculosis frequently contains so few acid-fast bacilli that they are difficult to detect.
For the improved detection of acid-fast bacilli from sputum sediments, author applied filter smear method by using membrane filter.
In this experiment, 138 sputvm sediments were digested with 5 percent sodium hypochlorite(clorox). Each digested sputum sediment was filtered by Millipore membrane filter(0.45㎍ in pore sige, 25mm in diameter) and filtered membrane was smeared on the serum coated slide glass for detecting acid-fast bacilli by microscopic examination. These results were compared with those of culture isolations and smears prepared from centrifugates and direct smears of same specimens.
The results were summarized as follows,
1. The number of positive specimens detected by culture isolation was 122(88.4%) and by microscopy was 103(74.6%) on direct smears and 116(84.1%) on centrifugate smears and 128(92.8%) on filter smears.
2. The average Gaffky number was 2.73 on direct smear, 3.92 on centrifugate smear and 5.20on filter smear.
3. Of 35 negative sputum specimenes by direct smears, 13(37,1%) were Positive on centrifugate smears and 25(71.4%) were positive on filter smears.
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